Deoxynivalenol Elisa has lower price but nearly the same quality compared to that made by European or USA.
1. INTENTED USE
Quicking Deoxynivalenol ELISA Kit is a direct method competitive enzyme-linked immunoassay for the quantitative detect the presence of Vomitoxin in samples of grains and feed.
2. PRINCIPLE OF THE ASSAY
Quicking Vomitioxin ELISA kit is a direct method competitive enzyme-linked immunoassay. The assay is performed in ELISA microwells, which have been precoated with anti-Vomitoxin antibodies. When standards or samples are added into the microwells, the free Vomitoxin antigens and the enzyme conjugate will compete to combine the antibody binding site on the microwells during incubation. After that, the unbound materials will be removed in a washing step. Then, two substrates are added into the microwells. The bound enzyme conjugate will convert the colorless substrate into blue. The color will change from blue to yellow after adding stop solution. Absorbance is then measured spectrophotometrically (450 nm) and colour intensity results are inversely proportional to the original Vomitoxin amount in the sample. Sample concentrations are then calculated on the basis of a calibration curve derived from the standards.
3. KIT COMPONENT
- 1 X Microtiter plate (8 microwells X 12 removable strips) precoated with anti-Vomitoxin antibodies
- 6 X Vomitoxin standard solutions (1mL each): 0 ng/mL,10 ng/mL, 100 ng/mL, 500 ng/mL, 2500 ng/mL
- 1 X Enzyme-linked conjugate (6 mL)
- 1 X Washing buffer concentrate (10 X , 50 mL)
- 1 X Substrate A: Carbamide Peroxide, (6 mL)
- 1 X Substrate B: TMB solution (6 mL)
- Product Manual
4. PREPARATION OF WORKING SOLUTIONS
5. SAMPLE PREPARATION
6. ELISA TESTING PROTOCOL
7. CALCULATION
A special program with Excel functionality, Quicking ELISA Analysis Program in Excel, is available for giving the ELISA test results.
8. STORAGE
- The kit should be stored at 2~8°C, NO FREEZING.
- Unused test wells should be sealed and dryly stored.
9. LIMITATION
Quicking Vomitoxin ELISA Kit is used as a screening system. If a more accurate result is required, other methods such as HPLC or GC/MS is suggested to give a confirmation.
Deoxynivalenol Elisa
