DON Test is a competitive immunoassay for the semi-quantitative detection of the presence of Deoxynivalenol.
DON Test (Feed)
Economical | Inexpensive first step in your testing protocol |
Simple | No special training or equipment required |
Fast | Results in as little as 5 minutes |
Convenient | Easily performed in the field or lab |
Precise | Limits of detection as low as 200 ppb |
Durable | Long shelf life |
Wide Range | 200 to 1000 ppb |
Simple sample preparation | Homogenization and extraction |
Reagents | Assay diluent (provided); distilled water (user provided) |
TEST PROCEDURE
- Assay diluent preparation (diluent factor 10).
- Grind a representative sample. Weigh out a 1.0 g sample into the centrifugal tube (15 mL).
- Add assay diluent. (REF: Table)
Cut-off (ppb) | 200 | 500 | 1000 |
Assay Diluent (mL) | 2.0 | 5.0 | 10.0 |
- Shake for 5 minute.
- Do a centrifugation at 4000 rpm for 1 min.
- Transfer 0.2 mL of supernatant into the centrifugal tube (1.5 mL).
- Add 0.8 mL of assay diluent.
- Place a cassette horizontally.
- Drop 3 drops into the assay sample hole.
- Interpret the result visually in 5-10 minutes.
INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone.
Negative: The presence of both bands in C zone and T zone.
Invalid: No band appears in C zone.
DON Test (Feed)
