... of pure semen with a micropipette, ( so we must do 2x0.2ml).We turn on the colorimeter (on ... ) and determine the value of reference (which will be 0,0)pressing the keyboard (zero).Next ,we ... interception of thses 2 values on the scale),which must be divided by the desires concentration of the doses ... , for example, we will decide that the semen must be deluted at 3m m SPZ(which corresponds not only at ...