... well strips into plate frames. Re-sealed the unused microplate, stored at 2 - 8 °C , not ... antibody working solution into each well, seal the microplate with the cover membrane, Mix gently by shaking ... shaking the platemanually. Set the wavelength of the microplate reader at 450 nm to determine the OD value ... reagents must avoid light exposure, and each microplate should be sealed by the cover membrane.5 ...