1. Brief This product is used for testing β-lactamase residue in raw milk. Sensitivity is as following Table 1, suitable for all kinds of enterprises and Inspection agency. Table 1: Relation between Incubation time and sensitivity Incubation Time Test Sensitivity 30min 3U 20min 5U 10min 10U 6min 20U 2. Components 1) β-lactamase colloidal gold rapid test strip (Including tablet, reagents board, dropper, desiccant) (20 pieces/kit) 2) Manuals (1 piece/kit) 3) 5 ml graduated tube (20 pieces/kit) 4) Dilution solution (2 tubes/kit) 3. Sample preparation 1). Open water bath, adjust to 37℃, begin the experiment when the water temperature reaching 37℃; 2). Take test tube rack into water bath, make water surface a little lower than the top of test tube rack; 3). Unseal the package ofβ-lactamase test strip, take out the small sealed bag, take the tablet into the 5 ml graduated tube; 4). Use pipette to take 1ml dilution solution and 0.25ml raw milk (or use the dropper drop 7 drops of raw milk) into the 5ml graduated tube within tablet, use dropper to mix fully of milk and dilution solution for 10s;(Before incubation, make sure tablet is fully immersion into milk) 5). Put the above 5ml graduated tube into test tube rack, incubation; (milk surface must lower than water surface) 6). Choose incubation time as above Table 1. 7). After incubation, use dropper to mix sample evenly for 10s, then take 6 drops of milk sample to test. 4. Operation procedures 1) Read the Manuals, return the reagents to room temperature (20℃~30℃) before testing. 2) Take out the test strip, put it on desk flatly, as the following picture on right (Use it immediately after opening the package.) 3) Use dropper absorb milk sample, vertically add 6 drops (about 200μL) into sample collecting well, timing after adding sample. 4) Read the result in 3-5 minutes, otherwise it is invalid.
