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Swine Foot and Mouth Disease IgG Antibody Clinical Chemistry Test Kit
Swine Foot and Mouth Disease IgG Antibody Clinical Chemistry Test Kit

Swine FMD IgG Antibody ELISA test kit 1.Sensitivity:qualitative 2.Spec:192 wells/kit 3.Sample:serum 4.Brand:Green Spring

Swine Foot and Mouth Disease IgG Antibody ELISA test kit

1.Introduction

The GreenSpring ™ FMD -Ab ELISA is developed to detect the O type antibodies of Porcine Foot and Mouth Disease in porcine serum samples and assess the immunity conditions against FMD in the pig farms.2. PrincipleThe kit is based on an indirect enzymatic immunoassay(Indi rect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other component s. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm).

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3. The kit components1 Antigen coated Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 pieces (192 wells)2 Negative control serum (green lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 tube (1 mL)3 Positive control serum (red lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 tube (1 mL)4 Anti-pig IgG-HRP conjugate(yellow lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 bottle (22 mL)5 20 × concentrated washing buffer (white lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 bottle (30 mL)6 Substrate A (purple lid)/B (yellow lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . (12 mL/each)7 Stop solution (blue lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 bottle (12 mL)8 Sample diluent (transparent lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 bottle (50 mL)4.Precautions and warnings for users

1. Read the use instructions carefully.2. Bring all reagents to room temperature prior to use.3. Do not use components after expiration dates and do not mix components from different lots.4. If the dry bag is red, don ’ t use.5. Don ’ t pollute any components.6. Reseal the plates after use and avoid to wet.

7. Do not pipette by mouth

Specifications: Specifications: Specifications: 96 wells × 2.Expiry Expiry Expiry date: date: date: 6 months.Storage: Storage: Storage: Storing at 4 °C , in the dark.

Swine Foot and Mouth Disease IgG Antibody Clinical Chemistry Test Kit

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