Detailed Product Description

  Enzyme Storage Buffer: AMV Reverse Transcriptase (AMV-RT) is supplied in 200mM potassium phosphate (pH 7.2 @4°C), 0.2% Triton® X-100, 2mM DTT and 50% glycerol.

Source: Purified from avian myeloblastosis virus particles.

Storage Conditions: Store at 20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.

See the expiration date on the Product Information Label.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide

into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3), 40mM KCl,

8.75mM MgCl

2, 10mM DTT, 0.1mg/ml acetylated BSA, 1mM radiolabeled dTTP and 0.25mM poly(A):oligo(dT). See the unit

concentration on the Product Information Label.