Shenzhen Lvshiyuan Biotechnology Co., Ltd. [China]

Neomycin ELISA test kit 1.Test Sensitivity: 0.5 ppb 2.Specifications : 96 wells 3.Brand: Green Spring 4.MOQ: 1kit

Neomycin ELISA test Kit

Catalog No. LSY-10005 

1. Principle

This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.5 ppb 

Detection limit

Honey............................................................................................. 10 ppb

Chicken/liver, pork/liver, fish, shrimp.................................................. 10 ppb

 Recovery rate

Chicken, pork, honey, fish, shrimp................................................... 85±15%

Liver (chicken or porcine)................................................................ 70±10%

Milk, milk powder ………………………………………………………….. 85±10%

Cross-reaction rate

Neomycin.......................................................................................... 100%

Streptomycin................................................................................... < 0.1%

Gentamicin...................................................................................... < 0.1%

Dihydrostreptomycin........................................................................ < 0.1%

Kanamycin...................................................................................... < 0.1%

Tobramycin...................................................................................... < 0.1%

Sisomicin........................................................................................ < 0.1%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody concentrated solution (7 mL).......................................... blue cap

5)   Substrate A solution (7 mL).......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   20× concentrated washing buffer (40 mL)..................................... white cap

9)   2× concentrated redissolving solution (50 mL)..................... transparent cap

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporter of the most advanced Food Safety ELISA test kit(e.g. Nitrofuran ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine Foot and Mouth Disease lgG Distinguish kit,PRRSV Antibody ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by DHL,EMS or other cheaper ways.

Neomycin ELISA test kit for testing drug residue in honey