Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH
Synonym | Aminomethyl idinetrimethanolTalatrolTHAM; TRIS |
Molecular Formula | C4H11NO3 |
Molecular Weight | 121.14 |
CAS Number | 77-86-1 |
Application
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode
Specification
Appearance (Color) | White |
Appearance (Form) | Crystalline Powder |
Solubility (Color) | Colorless |
Solubility (Turbidity) | Clear |
| at 200 g plus 300 ml of H2O |
UV Absorbance 290nm | ≤0.05 |
| 40% W/W |
Water (by Karl Fischer) | ≤0.2 % |
Iron (Fe) | ≤1 ppm |
Heavy Metal | ≤2 ppm |
Titration by H2SO4 | ≥99.9 % |
Melting Point | 168 - 172 °C |
Recommended Retest Period | 7years |
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Tris(hydroxymethyl)aminomethane
