1.EP standard Chymotrypsin 2.White crystallize powder 3.Assay: Not less than 5.0 ukatal/mg 4.Bovine or porcine pancrease
Chymotrypsin EP6.3
CAS 9004-07-3
Source Bovine (Porcine) pancreas
General description
Chymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).
Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.
The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Chromatography and ultra-filtration.
Items | Specification | Method |
Description | white crystallize powder | EP6.0 |
Identification | A) A purple colour develops B)No colour develops with in 3 min of mixing | EP6.0 |
Appearance of solution | Solution S is not more opalescent than reference suspension 2 | EP6.0 |
Absorbance | 1) 281nm : 18.5 ~ 22.5 2) 250nm : not more than 8.0 | EP6.0 |
PH | 3.0-5.0 | EP6.0 |
Histamine | Not more than 1µg/5.0µkatal | EP6.0 |
Loss on drying | Not more than 5% | EP6.0 |
Limit of trypsin | Not more than 1% | EP6.0 |
Assay | Not less than 5.0 ukatal/mg, calculated on the dried basis | EP6.0 |
Storage
Sealed, Dark, at temperature 2-8°C
Chymotrypsin EP6.0 CAS No. 9004-07-3
