... are characterized by the presence of antinuclear autoantibodies (ANA).Higher titers of ANA is highly indicative of , though they also ... connective tissue disorder, , & ,, , , and myasthenia gravis.ANA ELISA IMMUNOASSAY TEST KIT Purified ANA antigen is coated on the surface of microwells ... . Patient serum is added to the wells, and the ANA antibody, if present, binds to the antigen during incubation. ...